Glutamate and GABA dynamics in early ischaemia of rat hippocampal slices
نویسنده
چکیده
During brain ischaemia, the run-down of transmembrane ion gradients caused by the fall of ATP levels occurring leads to a rise in extracellular glutamate and GABA concentrations. The rise of glutamate concentration triggers neuronal death. In simulated ischaemia of hippocampal slices, taken from rats humanely killed in accordance with UK animal use legislation, we have used receptors in whole-cell patch-clamped CA1 pyramidal cells to sense released glutamate and GABA. ECl was set to 0 mV, so that currents mediated by ionotropic GABA receptors were inward, and membrane current was recorded at _30 mV to allow glutamate sensing by NMDA and AMPA receptors.
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